Journal: Life

Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

doi: 10.3390/life14101260

Figure Lengend Snippet: shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .

Article Snippet: Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

Techniques: shRNA, Knockdown, Expressing, Western Blot

Journal: Life

Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

doi: 10.3390/life14101260

Figure Lengend Snippet: DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.

Article Snippet: Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

Techniques: Microscopy

Journal: Frontiers in Oncology

Article Title: COL10A1-DDR2 axis promotes the progression of pancreatic cancer by regulating MEK/ERK signal transduction

doi: 10.3389/fonc.2022.1049345

Figure Lengend Snippet: COL10A1 regulates DDR2 expression in PDAC cells. (A) Western blot results showed the effect of COL10A1 on DDR2 and P-DDR2 protein levels. (B) IHC staining of DDR2 in PDAC and normal pancreatic tissues. The scale bar at 200× magnification represents 20 µm. The scale bar at 400× magnification represents 50 µm. (C) DDR2 IHC scores were differentially expressed between the normal and tumor groups. n = 30. Two-tailed Student’s t-test. (D) Pearson correlation analysis was conducted to analyze the relation between COL10A1 and DDR2 in 30 PDAC tissues. (E) Co-localization of COL10A1 and DDR2 in PDAC cells detected by immunofluorescence. (F, G) The detection of the interaction between COL10A1 and DDR2 by Co-IP. *** P <0.001.

Article Snippet: The following primary antibodies were used: DDR2, 1:200 (ABclonal); COL10A1, 1:200 (BOSTER); E-cadherin, N-cadherin, Vimentin, 1:200 (Cell Signaling Technology).

Techniques: Expressing, Western Blot, Immunohistochemistry, Two Tailed Test, Immunofluorescence, Co-Immunoprecipitation Assay

Journal: Frontiers in Oncology

Article Title: COL10A1-DDR2 axis promotes the progression of pancreatic cancer by regulating MEK/ERK signal transduction

doi: 10.3389/fonc.2022.1049345

Figure Lengend Snippet: COL10A1 facilitates the malignant biological behavior of PDAC cells through DDR2. (A) Effect of DDR2 overexpression on the proliferative capacity of SW1990, BXPC cells after disruption of COL10A1 using clone formation assay. (B) Effects of shCOL and shCOL10A1+DDR2 on the proliferation ability of SW1990 and BXPC-3 cells by CCK8 assay. (C-F) Effects of shCOL and shCOL10A1+DDR2 on the migration ability of SW1990 and BXPC-3 cells by wound healing assays. Scale bar, 50 µm. (G-I) Effects of shCOL and shCOL10A1+DDR2 on the migration ability of SW1990 and BXPC-3 cells by transwell assays. Scale bar, 50 µm. * P < 0.05, ** P < 0.01, **** P < 0.0001. ns, no significance.

Article Snippet: The following primary antibodies were used: DDR2, 1:200 (ABclonal); COL10A1, 1:200 (BOSTER); E-cadherin, N-cadherin, Vimentin, 1:200 (Cell Signaling Technology).

Techniques: Over Expression, Disruption, Tube Formation Assay, CCK-8 Assay, Migration

Journal: Frontiers in Oncology

Article Title: COL10A1-DDR2 axis promotes the progression of pancreatic cancer by regulating MEK/ERK signal transduction

doi: 10.3389/fonc.2022.1049345

Figure Lengend Snippet: COL10A1-DDR2 axis promotes EMT in PDAC cells through activation of MEK/ERK pathway. (A) GSEA validated EMT-related pathways in high COL10A1 expression PDAC cohorts of GSE15471 dataset. (B) The effect of COL10A1 on the expressions of EMT signaling proteins by Immunofluorescence. (C) The effect of COL10A1 and DDR2 on the expressions of EMT signaling proteins by Western blot. (D) Western blot detection of the effect of overexpression of DDR2 on protein expression changes in the EMT pathway induced by interference with COL10A1. (E) Western blot analyses of MEK/ERK pathway proteins in the indicated cells. (F) The protein expression of EMT pathway was detected by western blot in PDAC cells after treated with ERK inhibitor SCH772984. (G) H&E staining and IHC staining of COL10A1, DDR2, P-ERK, E-cadherin, N-cadherin and Vimentin in PDAC orthotopic models. Scale bars, 20 μm.

Article Snippet: The following primary antibodies were used: DDR2, 1:200 (ABclonal); COL10A1, 1:200 (BOSTER); E-cadherin, N-cadherin, Vimentin, 1:200 (Cell Signaling Technology).

Techniques: Activation Assay, Expressing, Immunofluorescence, Western Blot, Over Expression, Staining, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: COL10A1-DDR2 axis promotes the progression of pancreatic cancer by regulating MEK/ERK signal transduction

doi: 10.3389/fonc.2022.1049345

Figure Lengend Snippet: Model: COL10A1 activated the MEK/ERK signaling pathway of pancreatic cancer cells and promoted the EMT of pancreatic cancer cells through a DDR2-dependent pathway.

Article Snippet: The following primary antibodies were used: DDR2, 1:200 (ABclonal); COL10A1, 1:200 (BOSTER); E-cadherin, N-cadherin, Vimentin, 1:200 (Cell Signaling Technology).

Techniques:

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 1. (a) Schematic diagram showing the structure of full-length DDR2, human DDR2-Fc, and mouse DDR2-V5-His constructs. SS, signal sequence; DS, discoidin domain; ECD, extracellular domain; IJXM, intracellular juxtamembrane region; TMD, transmembrane domain; ICD, intracellular domain; KD, kinase domain. (b) Purified recombinant DDR2-V5-His and DDR2- Fc proteins (20 ng/lane) were resolved by SDS-PAGE under reducing conditions (+βME), either with 4%–12% (w/v) Bis–Tris gels (left panel) or under reducing and non-reducing (−βME, 100 ng/lane) conditions with 10% SDS-PAGE (right panel). The separated protein was detected by immunoblotting using anti-epitope or anti-DDR2 antibodies as indicated.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Construct, Sequencing, Purification, Recombinant, SDS Page, Western Blot

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 2. (a) Solid-phase binding of DDR2 ECD proteins to immobilized bovine-dermal collagen I as indicated. Binding was detected using antibodies against DDR2 ECD. (b) Inhibition of fibrillogenesis of bovine-dermal collagen I assessed using turbidity measurements. DDR2 ECD proteins (40 μg/ml) as indicated were incu- bated with 200 μg/ml of neutralized collagen I in 96-well plates at 37 °C.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Binding Assay, Inhibition

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 3. AFM height images of monomeric DDR2-V5-His and dimeric DDR2-Fc before and after binding to bovine-dermal collagen I as indicated (a–d). DDR2-V5-His and DDR2-Fc particles bound to collagen are indicated by black and white arrows, respectively. Particle size distribution and average sizes are indicated in the accompanying histograms in panels e and f and in Table 1.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Binding Assay

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 4. Live cell imaging of DDR1b-YFP- (a–d) and DDR2-GFP- (e–g) expressing MC3T3-E1 cells using wide-field fluorescence microscopy, before and after the addition of collagen “C” as indicated. Insets (in panels a–g) show selected regions, which have been magnified from the corresponding images to visualize receptor assemblies. The location of these selected regions on the cell surface is indicated by dashed boxes. Endogenous DDR expression in MC3T3-E1 cells was evaluated using Western blotting panel h. Lysates of MC3T3-E1 cells were resolved by reducing 7.5% SDS-PAGE followed by immunoblot analyses using the indicated DDR antibodies. Asterisk (*) shows a non-specific band. Fluorescence microscopy images (a–c) show that DDR1b-YFP exhibits a uniform distribution on the cell surface before collagen stimulation and results in cluster formation upon collagen stimulation. Quantitative analysis (i) indicates that the number of punctuate structures in cells significantly increases upon collagen stimulation (*p b 0.05) and persist at 4 h. After 4 h of collagen stimulation, a subpopulation of DDR1b-YFP-expressing cells also exhibits the presence of long, filamentous structures (d). DDR2-GFP exhibits a uniform distribution on the cell surface before collagen stimulation (e) and does not result in cluster formation upon collagen stimulation (f). However, at 4 h post-collagen administration, filamentous structures were also observed in DDR2-GFP-expressing cells (g). The distribution of uninterrupted contour length of filamentous structures formed in DDR1b-YFP- and DDR2-GFP-expressing cells after 4 h of collagen stimulation is shown in panel j.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Live Cell Imaging, Expressing, Fluorescence, Microscopy, Western Blot, SDS Page

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 6. ICC performed on non-permeabilized cells for evaluating the association of collagen I with filamentous structures formed in (a) DDR1b-YFP- and (b) DDR2-GFP-expressing cells after 4 h of collagen stimulation. Total receptor is indicated in YFP channel (green), while staining with collagen antibodies is shown by TRITC (red). Co-localization YFP and TRITC is shown in yellow. Blue represents nuclear (DAPI) staining. Insets (in first row of each panel) show selected regions, which have been magnified from the corresponding images. Selected regions from three different cells are shown in the second row in each panel. YFP/GFP-positive filamentous structures had a very similar morphology as collagen fibrils and were observed to anchor the fibrils at cell edges. Collagen staining was intermittently present on or interspersed with YFP/GFP- positive filamentous structures.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Expressing, Staining

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 7. Western blotting of DDR expression and phosphorylation in MC3T3 cells. MC3T3-E1 cells were transiently transfected with (a) DDR1b-YFP or (b) DDR2-GFP expression vectors and stimulated with 20 μg/ml collagen I (+), as described in Materials and Methods. After 4 h of collagen stimulation, the cells were lysed in RIPA buffer and equal protein concentrations (25 μg/lane) were resolved by reducing 7.5% SDS-PAGE followed by immunoblot analyses using the indicated antibodies to DDR1 or DDR2. β-Actin was used as loading control. Number 1 in panel a indicates an additional band detected with D1G6 antibodies (discussed in the Results section). Asterisks (*) show non-specific bands.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Western Blot, Expressing, Phospho-proteomics, Transfection, SDS Page, Control

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Fig. 10. ICC performed on permeabilized cells for evaluating spatial distribution of receptor phosphorylation in DDR2- GFP-expressing cells after (a) 30 min and (b) 4 h of collagen stimulation. Total receptor is indicated in GFP channel (green), while staining with pDDR2 (Y740) antibodies is shown by TRITC (red). Co-localization GFP and TRITC is shown in yellow. Blue represents nuclear (DAPI) staining. Insets (in top row in each panel) show selected regions, which have been magnified from corresponding images. Little to no co-localization signal was detected, 30 min after collagen stimulation (a). At prolonged collagen stimulation (4 h), a number of filamentous structures formed in DDR2-GFP expressing cells co-localized with Y740 signal (b). The second row in panel b consists of selected regions from three different cells showing co-localization of filamentous structures with Y740 signal. A weak signal for Y740 (arrows) without a corresponding GFP signal can be observed in insets in (b, top row), which could correspond to endogenous pDDR2.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Phospho-proteomics, Expressing, Staining

Journal: Journal of molecular biology

Article Title: Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I.

doi: 10.1016/j.jmb.2018.11.015

Figure Lengend Snippet: Scheme 1. Postulated model for spatial distribution and phosphorylation of DDRs upon collagen stimulation. DDR1 and DDR2 exist as homodimers on the cell surface. (a) Upon addition of monomeric collagen I, DDR1 and DDR2 interact with collagen, which leads (arrow 1) to the assembly of the receptors into filamentous structures aligned with collagen fibrils. This process occurs at prolonged (~4 h) times after collagen I stimulation. Phosphorylation of both DDR1 and DDR2 (arrow 2) co- localizes in these structures, as determined using Y513 (pDDR1b) as well as Y792 (pDDR1) and Y740 (pDDR2) antibodies, indicated as black and green circles. (b) In another pathway, DDR1b assembles into clusters within minutes of collagen I stimulation (likely binding to non-fibrillar collagen present at early stages of fibrillogenesis) and gets endocytosed. However, phosphorylation at Y513 and Y792 is not detected in these clusters at the early (~30 min) time point. At later times (~4 h) post- ligand administration, phosphorylation of DDR1b in its IJXM (Y513) (green circles) but not in its KD (Y792) is detected in the DDR1b clusters. At present, it is not clear if Y513-positive clusters are present on the cell surface or constitute pools of endocytosed DDR1b receptors localizing within endosomes. Owing to the fact that DDRs contain numerous Tyr residues within the IJXM region and their KDs, phosphorylation at other Tyr residues (indicated by the red dashed circles) may also be ensuing in the structures described in panels a and b but are undetectable by the tools available at this time. It is likely that formation of higher-order assemblies of DDRs may recruit additional cytosolic proteins, which mediate specific cellular processes.

Article Snippet: Mouse monoclonal (MAB25381) and goat polyclonal (AF2538) anti-DDR2 antibodies (raised against a peptide sequence in the DDR2 ECD) and rabbit monoclonal against phospho-DDR2 (DDR2-Y740, catalog no. MAB25382) were obtained from R&D Systems, Minneapolis, MN.

Techniques: Phospho-proteomics, Binding Assay

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Representative image of DDR2 and collagen type I immunostaining in human breast cancer. ( A – C ): immunostaining of DDR2 in breast cancer cells ( A ), normal breast epithelium ( B ), and human heart as a positive control of DDR2 ( C ). ( D – F ): immunostaining of collagen type I in cancerous stroma ( D ), normal breast stroma ( E ), and human skin as a positive control of collagen type I ( F ). Bar = 100 µm, respectively.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Immunostaining, Positive Control

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinicopathological factors in 224 breast carcinomas.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinicopathological factors according to collagen type I status in 224 breast carcinomas.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinical outcomes of breast cancer patients (n = 224). ( A – D ): disease-free survival ( A , C ) and breast cancer-specific survival ( B , D ) according to DDR2 status ( A , B ) or DDR2/collagen type I combination status ( C , D ). ( E – H ): disease-free survival according to DDR2 status ( E , F ) or DDR2/collagen type I combination status ( G , H ) in the patients who received chemotherapy ( E , G ) or not ( F , H ).

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Uni- and multivariate analysis of disease-free survival.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 in the proliferation of human breast cancer cell lines in the presence of collagen type I. ( A ) Immunoblotting of exogenous DDR2 protein in MCF-7, MDA-MB-231, and T47D cells. ( B – D ): cell proliferation of MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( D ) transfected with an empty vector or DDR2-expressing vector in the absence or presence of collagen coating. ( E ) Immunoblotting of DDR2 in the cells transfected with siRNA against DDR2 (siDDR2-1, 2). ( F – H ): cell proliferation of MCF-7 ( F ), MDA-MB-231 ( G ), and T47D ( H ) transfected with siRNAs in the presence of collagen coating. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the empty vector, respectively.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 on the resistance to epirubicin in the breast cancer cell lines. ( A – C ): viability of MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( C ) transfected with an empty vector or DDR2-expressing vector under epirubicin treatment (500 nM for MCF-7; 250 nM for MDA-MB-231 and T47D). These cells were plated onto collagen-coated culture plates. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the empty vector, respectively. ( D – F ): viability of MCF-7 ( D ), MDA-MB-231 ( E ), and T47D ( F ) transfected with siRNA targeting DDR2 under epirubicin treatment. ( G , H ) mRNA and protein expression in chemosensitive parental cells and epirubicin-resistant cells ( G ; MCF-7 series, H ; MDA-MB-231 series). ** p < 0.01, respectively. ( I , J ) The effect of DDR2 inhibitor WRG-28 treatment (48 h) on the proliferation of chemosensitive parental cells and epirubicin-resistant cells ( F ; MCF-7 series, G ; MDA-MB-231 series).

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Transfection, Plasmid Preparation, Expressing

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 on the apoptosis of breast MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( C ). *** p < 0.001.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: Features of Individual 1, with the p.Leu610Pro DDR2 Variant

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques: Variant Assay

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: Overview of Phenotypic Features

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques: Variant Assay

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: DDR2 Structure and Amino Acid Conservation

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques:

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: Autophosphorylation of DDR2 and Effect of Dasatinib Treatment

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques: